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. 2004 Sep;78(18):9750–9762. doi: 10.1128/JVI.78.18.9750-9762.2004

FIG. 7.

FIG. 7.

Production of HCMV is inhibited in S2 and S3 cells infected with HCMV. Cells were mock or HCMV infected at an MOI of 1 PFU/cell. (A) Inhibition of the development of cytopathic effect in S2 and S3 cells. Panels: 1 to 3, control P7 cells; 4 to 6, UL76-expressing S2 cells; 7 to 9, S3 cells. The progress of cytopathic effect was recorded at 7 and 14 days postinfection with HCMV. (B) Titration of HCMV production on P7 (open bars), S2 (hatched bars), and S3 (shaded bars) cells at 3, 7, 14, and 21 days postinfection. (C) The accumulation of HCMV DNA is delayed and reduced in virus-infected S2 and S3 cells. Total DNA was harvested from infected cells at 1, 3, 5, and 7 days postinfection (dpi). The viral DNA was analyzed by slot blotting and probed with the IE1 cDNA. As a control, the blot was stripped and reprobed with the GAPDH gene.