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Western blot of the His6-tagged envelope proteins in cell lysates. The proteins were deglycoslated and probed with an anti-SU antibody, goat anti-Rauscher MuLV gp69/71 at a 1:1,000 dilution as primary antibody, and a rabbit anti-goat IgG HRP conjugate as second antibody at a 1:10,000 dilution. Pr85 is the precursor form of the envelope proteins, and SU is the mature form of the envelope proteins.
