TABLE 1.
Binding affinity of retrovirus vectors for Ni-NTA agarose
| Fraction | Viral titer (105 CFU/ml)a
|
||
|---|---|---|---|
| Ecotropic MuLV | E/A-PRR | RVHis_e | |
| Feed | 4.5 ± 0.3 | 4.3 ± 0.3 | 3.5 ± 0.5 |
| Flowthrough | 4.0 ± 0.5 | 4.4 ± 0.1 | 0.042 ± 0.004 |
a
Each titer is the average of three independent experiments ± the standard error. Each fraction contained 3 ml of the vector supernatant. E/A-PRR, the viruses enveloped with an ecotropic MuLV Env protein with the PRR region replaced by that of an amphotropic virus. RVHis_e: the virus enveloped with His6-tagged ecotropic MuLV Env.