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. 2004 Sep;78(18):9820–9827. doi: 10.1128/JVI.78.18.9820-9827.2004

TABLE 5.

Elution of His6-tagged retrovirus vectors under different pHs

Fraction (3 ml) Viral titer (105 CFU/ml) Proteins (mg/ml) DNA (μg/ml)
Feed 4.2 7.72 0.16
Flowthrough 0.03 2.13 0.11
pH 7.3a 0 0.89 0.059
pH 6.5 0 1.11 <2.5 × 10−5
pH 6.0 0 1.02 <2.5 × 10−5
pH 5.5 0 0.57 <2.5 × 10−5
pH 5.0 0 0.15 <2.5 × 10−5
a

The pH value of the elution buffer.