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. 2004 Sep;78(18):10023–10033. doi: 10.1128/JVI.78.18.10023-10033.2004

FIG. 5.

FIG. 5.

Rescue in trans of HUVEC tropism and subsequent leukocyte transfer. (A) Analysis of viral growth showing the median number of CPE plaques per 24-well HUVEC culture (three wells examined) either expressing the void vector and infected with RVFIX or expressing the individual UL131, UL130, or UL128 genes and infected with the relevant KO (Δ) or natural virus mutant (mut). No plaque was observed after infection of HUVECs expressing the void or the heterologous vector with deletion or natural mutants. (B) Median number of pp65-positive PMN (□) and monocytes (MN, ▪) after coculture with 24-well HUVEC monolayers (three wells examined) expressing individual UL131, UL130, or UL128 genes and infected with the homologous KO or natural virus mutant at 10 days p.i. Transfer of pp65 to leukocytes occurs only at the late stage of productive virus replication (12). (C) Virus growth in HUVECs transduced with the UL128 gene and infected with RVFIXΔUL128K (a and b) or Merlin strain (UL128 mutant) (d and e). Panels a and d show gB+IE antigen-positive plaques; panels b and e show CPE plaques (arrows). In panels c and f, pp65-positive leukocytes after coculture with HUVECs transduced with UL128 and infected with RVFIXΔUL128K (c) or Merlin strain (f) (10 days p.i) are shown.