TABLE 2.
Rescue in cis of both EC tropism and leukocyte transfer of Huv− Leuk− viral variants showing reversal of UL131-128 mutations after adaptation to growth in HUVECs
| HCMV strain (mutated gene) | Rescue in cis of HUVEC tropism and leukocyte transfer after attempts at adaptation to growth in HUVECs (Huv+ Leuk+ rev) in:
|
|
|---|---|---|
| HUVECa | PMN/monocytesb | |
| RVFIX mutants | ||
| RVFIXΔUL132-128 | 0 | 0/0 |
| RVFIXΔUL131K | 0 | 0/0 |
| RVFIXΔUL130 | 0 | 0/0 |
| RVFIXΔUL128K | 0 | 0/0 |
| Clinical isolates | ||
| VR1814 Huv− Leuk− (UL131) | 80 | 8,800/8,200 |
| VR3480 Huv− Leuk− (UL130) | 80 | 7,000/7,300 |
| Merlin Huv− Leuk− (UL128) | 80 | 7,500/8,000 |
| Laboratory strains | ||
| AD169 Huv− Leuk− (UL131) | 85 | 70/7,500 |
| Towne Huv− Leuk− (UL130) | 85 | 8,500/9,200 |
| Toledo Huv− Leuk− (UL131-128) | 0 | 0/0 |
a
That is, the percentage of infected cells in the HUVEC monolayer after more than 10 passages.
b
That is, the median number of pp65-positive leukocytes/2 × 105 leukocytes examined in five independent experiments. Individual figures were within a 30% range.