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. 2004 Sep;78(18):10023–10033. doi: 10.1128/JVI.78.18.10023-10033.2004

TABLE 2.

Rescue in cis of both EC tropism and leukocyte transfer of Huv Leuk viral variants showing reversal of UL131-128 mutations after adaptation to growth in HUVECs

HCMV strain (mutated gene) Rescue in cis of HUVEC tropism and leukocyte transfer after attempts at adaptation to growth in HUVECs (Huv+ Leuk+ rev) in:
HUVECa PMN/monocytesb
RVFIX mutants
    RVFIXΔUL132-128 0 0/0
    RVFIXΔUL131K 0 0/0
    RVFIXΔUL130 0 0/0
    RVFIXΔUL128K 0 0/0
Clinical isolates
    VR1814 Huv Leuk (UL131) 80 8,800/8,200
    VR3480 Huv Leuk (UL130) 80 7,000/7,300
    Merlin Huv Leuk (UL128) 80 7,500/8,000
Laboratory strains
    AD169 Huv Leuk (UL131) 85 70/7,500
    Towne Huv Leuk (UL130) 85 8,500/9,200
    Toledo Huv Leuk (UL131-128) 0 0/0
a

That is, the percentage of infected cells in the HUVEC monolayer after more than 10 passages.

b

That is, the median number of pp65-positive leukocytes/2 × 105 leukocytes examined in five independent experiments. Individual figures were within a 30% range.