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. 2004 Sep;78(18):9965–9976. doi: 10.1128/JVI.78.18.9965-9976.2004

FIG. 3.

FIG. 3.

Apoptin does not associate with SV40 LT. (A) No co-IP between apoptin and LT. pCMV-VP3 was transfected in SV40-stably transformed VH/SV cells or cotransfected with pRSV-LT in Saos-2 cells. Co-IP and Western blot assay were carried out 2 days posttransfection with apoptin antibody α-VP3-C or SV40 LT antibody 416. Lysates (Lys) without IP were used as control to show the coexpression of apoptin and SV40 LT. (B) No colocalization in the nucleus between apoptin and LT. Confocal immunofluorescence microscopy was carried out with apoptin- and LT-cotransfected primary human normal cell types VH10 and FSK-1 and with apoptin-transfected SV40 stably transformed cell types VH/SV and SVK-14. Apoptin was probed with α-VP3-C (green), and SV40 LT with 419 (red).