Figure 2.

P-selectin binding and expression of VLA-1 by circulating melanoma-specific CD8⁺ T cells correlate with survival of vaccinated patients. (A) Representative histograms illustrating highly differential expression of VLA-1 (patient numbers LAU205 and LAU1164) and binding to P-selectin (patient numbers LAU1129 and LAU1164) by Melan-A-specific CD8⁺ T_EM cells. (B,C) Correlation between expression of VLA-1 (B) or P-selectin binding capacity (C) of Melan-A-specific CD8⁺ T_EM cells and overall patient survival. (D,E) Kaplan–Meier overall survival analysis of patients with Melan-A-specific effector memory CD8⁺ T cells displaying greater than (“high”) or less than (“low”) median expression of VLA-1 (D) or P Selectin binding (E). (F,G) Kaplan–Meier disease-free survival analysis based on the T cell’s “high” or “low” expression of VLA-1 (F) or P-selectin binding (G). (H,I) Expression of VLA-1 (H) or P-selectin binding (I) by total or Melan-A-specific CD8⁺ T_EM cells from patients with greater than or less than median overall survival. (B,C) Spearman’s rank correlation coefficient. (D–G) Log-rank (Mantel–Cox) test. (H,I) **P < 0.01 (two-tailed Mann–Whitney test).