Figure 3.

CD8⁺ T cells expressing VLA-1 and other integrins are highly enriched in human melanoma metastases compared to blood circulation. (A) Representative dot plot illustrating the predominantly T_EM differentiation status of the CD8⁺ TIL analyzed in this study. (B) Representative histograms and quantification of P-Selectin binding by CD8⁺ T_EM cells isolated from blood circulation and melanoma tumors. (C) Representative dot plots of VLA-1 and CD103 expression on CD8⁺ T_EM cells derived from melanoma metastases in skin, lung, and brain, blood circulation, metastatic lymph nodes (TILN), and tumor-derived CD4⁺ T cells. (D) Representative dot plot showing expression of T_RM markers, CD69 and CD103, by VLA-1-expressing TIL. (E) Quantification of cells co-expressing VLA-1 and CD103 in the cellular compartments indicated. Numbers on FACS plots represent percentage cells in each gate (A,C). (D) **P < 0.01, ***P < 0.005 (One-way ANOVA, Tukey’s multiple comparisons test).