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. 2016 Dec 12;7:573. doi: 10.3389/fimmu.2016.00573

Figure 4.

Figure 4

In subcutaneous murine tumors, CD8+ TIL display an integrin-expression profile that is unique to the tumor microenvironment and is dependent on subcutaneous immunization. (A) Representative dot plots of VLA-1 and CD103 expression by OT-1 T cells derived from tumor, tumor-draining lymph node (dLN), and spleen of mice bearing subcutaneous B16-Ova tumors on day 14 or day 21. (B) Quantification of co-expression of VLA-1 and CD103 by endogenous CD8+ T cells derived from dLN, tumor, or OT-1 T cells derived from tumor. (C) Representative dot plot of VLA-1 and CD103 expression by endogenous CD8+ T cells derived from B16-Ova tumors. (D) Representative dot plot showing co-expression of VLA-1 and CD103 by OT-1 T cells derived from a subcutaneous E.G7. Numbers on FACS plots indicate percentage positive cells in each gate (A,C,D). (B) ****P < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test).