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. 2016 Dec 12;6:38976. doi: 10.1038/srep38976

Figure 3. DISHEVELLED and PDZ-RGS3 are not in the EFNB3 reverse signaling pathway in VSMCs.

Figure 3

Experiments in this figure were repeated more than twice, and representative data are shown. (A) Effective mRNA knockdown of Disheveled and PDZ-RGS3 by siRNA. VSMCs from female WT mice were transfected with siRNAs of Disheveled and PDZ-RGS3 as described in Fig. 2. The mRNA expression of each gene was determined by RT-qPCR. The data are expressed as means ± SD of the ratios of the target gene signal versus the β-actin signal. The data were analyzed by Student’s t test. *p < 0.05. (B) Dishevelled and PDZ-RGS3 knockdown by siRNAs had no effect on WT VSMC contractility. Contractility of VSMCs from female WT mice were assessed in the presence of EFNB3 reverse signalling and DISHEVELLED/PDZ-RGS3 knockdown, as described in Fig. 2B. The data were analyzed with one-way ANOVA but no significant difference was observed.