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. 2016 Dec 12;6:38979. doi: 10.1038/srep38979

Figure 3. The involvement of autophagy in DHA inhibition of catabolic genes in chondrocytes under inflammatory conditions for 24 hours.

Figure 3

Representative images are shown. (A,B) The expression of ATG5 in chondrocytes 48 hours after ATG5-siRNA transfection was analyzed by Western blotting, and the optical density of ATG5/β-actin was analyzed. Data represent mean ± 95% CI, *P < 0.05, **P < 0.01, n = 6. (C,D) Western blotting analysis of LC3 levels in chondrocytes after ATG5 silence. Data represent mean ± 95% CI, *P < 0.05, **P < 0.01, n = 6. (E) AO staining for acidic vesicular organelles in chondrocytes 48 hours after transfection with ATG5 siRNA. F. 48 hours after ATG5 silencing, mRNA levels of MMP-3 and -13, ADAMTS-4 and -5, CCL-2 and -5, and CXCL1 in TNF-α- and/or DHA-treated chondrocytes were analyzed by real-time PCR. Data represent as mean ± 95% CI, *P < 0.05, **P < 0.01, n = 6.