Figure 5. Involvement of the NF-κB pathway in autophagic activation in chondrocytes under inflammatory conditions for 24 hours.

(A,B,C) Chondrocytes were treated with the TNF-α and/or NF-κB pathway inhibitor SM7368, and Western blotting analysis of IκBα and LC3 expression was done. The optical density of IκBα/β-actin and LC3-II/β-actin was analyzed. Data represent mean ± 95% CI, **P < 0.01. (D) The autophagic level in chondrocytes was analyzed by GFP-LC3 assay.