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. 2016 Dec 12;6:38792. doi: 10.1038/srep38792

Figure 2. EphB4 downregulation combined with ionizing radiation enhances the DNA damage response in HNSCC cells.

Figure 2

p-H2AX analysis shows a significant enhancement in the percentage of p-H2AX positive MSK-921 cells following EphB4 knockdown and ionizing radiation as demonstrated by immunofluorescence staining (A and B). In addition, an increase in Rad51-foci is also evident in MSK-921 cells (C and D). A similar trend was observed in Fadu cells in terms of p-H2AX (E and F) and Rad51 (G and H) expression. Data shown represent mean ± standard error from two to three independent experiments. *p < 0.05; **p < 0.005. Total magnification: 600–1000x. Western blot analyses demonstrate enhanced levels of p-H2AX, Ku80, and Rad51 indicative of the DNA damage response in the combination group compared to the other experimental groups in MSK-921 cells (I) and Fadu cells (J).