FIG. 2.

Knockdown of ILK in zebra fish. Morpholinos were injected into one-cell-stage zebra fish embryos as described in Materials and Methods. Patterning was investigated by using the morpholino in fish transgenic for GCFP under the direction of the flk-1 promoter. Fluorescence microscopy reveals loss of the normal regular network of intersegmental and parachordal vessels for a variable number of thoracic segments, usually extending caudally from the second or third somite. Other regions of the intersegmental vasculature exhibited patterning defects as described in the text. WT, wild type.