Table 1.

Representative kinetic parameters for PagF with various substrates run in triplicate and volumes occupied by those substrates in the PagF crystal structures

Substrate	Km, mM	V/K, min−1 mM−1*	Volume, Å3
l-Tyr	16	1.1	N/A
N-acetyl-l-Tyr	12	1.0	N/A
N-boc-l-Tyr	3.7	1.8	259
Tyr-Tyr-Tyr	0.14	39	453
cyc[INPYLYP]	2.15 × 10−4	3.4 × 104	792

See SI Appendix, Table S3 for full parameters and SI Appendix, Fig. S10 for curves used to generate the parameters. N/A, not determined.

^*Measured at an enzyme concentration of 1 mM PagF.