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. 2016 Nov 21;113(49):E7900–E7907. doi: 10.1073/pnas.1617168113

Fig. 4.

Fig. 4.

Immunogold labeling of fossil feathers (sample 201) compared with feathers of G. gallus. (A–C) Negative control of D–F, when the primary antiserum is omitted. (B) High-magnification image of the boxed area in A. (C) High-magnification image of the boxed area in B. (D–I) In situ nanogold immunohistochemistry of Eoconfuciusornis feathers exposed to anti-feather antibody. (E) Melanosomes from the same section of D and F; no nanogold particles were seen to specifically bind. (F) High-magnification image of the boxed area in D; arrowheads show binding of the nanogold beads. (G–I) High-angle annular dark-field (HADDF) images show binding of the nanogold beads from the same section of D–F. (H) High-magnification image of the boxed area in G. (I) High-magnification image of H; arrowheads show the filaments at the bead-binding regions. (J–L) In situ nanogold immunohistochemistry of G. gallus feathers exposed to anti-feather antibody. Nanogold particles specifically bind the keratinous tissues but do not bind the melanosomes, visualized in cross-section. (K) High-magnification image of the boxed area in J. (L) High-magnification image of the boxed area in K. (Scales are as indicated.)