Fig. 2.
Requirement of Kupffer cells for the Dectin-2–mediated antitumor system against liver metastasis. (A) Dectin-2 expression on liver-residing cells was analyzed with flow cytometry. Plots gated on CD45+ cells are shown. (B and C) WT and Dectin-2 KO mice were treated with control (ctrl) liposomes or clodronate liposomes 2 d before and after intrasplenic inoculation of SL4 cells (2 × 105 cells). On day 10, the livers were collected. Macroscopic images of the liver (B) and liver weights (C) are shown. (Scale bar: 1 cm.) Data are displayed as mean ± SEM. **P < 0.01. N.S., not significant.