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. 2016 Nov 21;113(49):14097–14102. doi: 10.1073/pnas.1617903113

Fig. S4.

Fig. S4.

Analysis of cancer cell recognition by Dectin-2 and Dectin-2–mediated gene induction in response to cancer cells. (A) Binding of sDectin-2 to SL4 cells and zymosan particles was analyzed by flow cytometry. Representative histograms are shown. (B) Kupffer cells (1 × 105 cells) isolated from WT and Dectin-2 KO mice were cocultured with or without 0.25 × 105 CFSE-labeled SL4 cells pretreated with N-glycosidase or a combination of O-glycosidase and neuraminidase (10), and 2 h later, CFSE intensity in CD45+ CD11b+ F4/80+ cells was analyzed by flow cytometry. Representative histograms of CFSE levels and proportion of CFSE+ cells are shown. (C) Kupffer cells (1 × 105 cells) and SL4 cells (1 × 105 cells) were cultured together or separately, and mRNA levels of Il6, Il23a, Cxcl1, and Ccl2 at the indicated time points were quantified by qRT-PCR analysis. (D and E) Kupffer cells (1 × 105 cells) were isolated from WT and Dectin-2 KO mice and cultured together with or separately from SL4 cells (1 × 105 cells). Eight hours later, mRNA expression levels of Il6, Il23a, Cxcl1, and Ccl2 (D) or Tnf, Arg1, and Cd206 (E) were analyzed by qRT-PCR. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01. N.S., not significant.