Fig. 4.

shRNA knockdown of MYC ESE eRNAs reduces MYC expression and ESE H3K27ac. (A) MYC mRNA expression levels from LCLs transduced with shRNA targeting MYC ESE eRNAs or control shRNA were determined by qRT-PCR. Expression levels were normalized against GAPDH, which were set to 1. (B) LCLs transduced with shRNA targeting MYC ESE eRNAs or control shRNA were fixed with 1% formaldehyde and lyzed. After sonication and dilution, lysates were precipitated with antibody against H3K27ac or control antibody overnight. Captured DNAs were reverse cross-linked and purified with Qiagen PCR purification column. qPCR was used to quantitate the precipitated DNA. A standard cure of input DNA was used to determine the enrichment. Error bar indicated SD from three experiments. *P < 0.05.