Fig. S2.

shRNA knockdown of ESE eRNAs decreases eRNA level and induces LCL growth arrest. (A) qRT-PCR was used to determine the levels of MYC ESE eRNAs following shRNA knockdown. qPCR was normalized against GAPDH, and the level in LCLs treated with shControl was set at 1. Error bars indicated SE. (B) Cell cycle distribution of LCLs transduced with control shRNA or shRNA targeting MYC ESE eRNAs. Three days after puromycin selection, cells were fixed and stained with propidium iodide. Cell cycle profiles were acquired by using FACS. (C) Western blot analysis of MYC expression in LCL cells treated with control shRNA or shRNA against SE428 or SE525 eRNA. GAPDH blotting was used as loading controls.