FIG. 5.

Position and extent of the RAD-51-positive region (black bars) in the gonads (shaded bars) of zhp-3 and wild-type animals. For each, 10 gonads were measured from the distal tip cell (DTC) to the end of continuous cell rows (roughly coinciding with the end of the pachytene zone) and normalized, and the borders of the transition zone and the RAD-51-positive region were entered. This region strikes on visual inspection by the abundance of RAD-51 foci. In quantitative terms it is defined as the zone in which a majority of nuclei have more than two prominent spots by RAD-51 immunostaining. The open and solid arrowheads indicate the distal and proximal borders of the transition zone, respectively (for a classification of meiotic stages in the gonad, see reference 23).