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. 2004 Sep;24(18):7998–8006. doi: 10.1128/MCB.24.18.7998-8006.2004

FIG. 6.

FIG. 6.

Immunostaining of GFP-tagged ZHP-3 (green) in the wild type and various mutants. In the wild type in nuclei of the transition zone (a), ZHP-3 forms spots (with major spots colocalizing with SYP-1, shown in red), while in pachytene nuclei (b) it appears as lines. In diakinesis (c) it is dispersed between the chromosomes. (d) Upon RNAi depletion of the SC-associated protein HIM-3, the linear organization of ZHP-3 is lost, and it colocalizes with the synapsis protein SYP-1 to one to several spots (orange, resulting from the mixing of green ZHP-3 and red SYP-1 markers). (e) No ZHP-3 lines are present in nuclei devoid of SYP-1, whereas unsynapsed axial elements in SYP-1-depleted nuclei are delineated by staining of REC-8 (f, red). (g) In spread SC preparations of the wild type, ZHP-3 (green) colocalizes with SYP-1 (red) on SCs, but the ZHP-3 pattern is discontinuous and faint, possibly due to an only weak association of ZHP-3 with SCs. Chromatin is stained blue by DAPI in all pictures. (h) In a spo-11 mutant, the pachytene linear organization of ZHP-3 is not affected. Bars, 5 μm.