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. 2004 Sep;24(18):8210–8220. doi: 10.1128/MCB.24.18.8210-8220.2004

FIG. 2.

FIG. 2.

Heterochromatin is not limited to tandem repetitious sequences. The phenotype of P[hsp26-pt, hsp70-w] transgenes indicates that while domains of euchromatin are present, the fourth chromosome is largely heterochromatic. The map of the fourth chromosome (top) (regions 101F to 102F) shows the positions of known and predicted genes and of transposable elements (upper row, 1360 elements; lower row, others) (15). Solid triangles and dotted triangles mark P element insertion sites that result in a solid red eye or induce a variegating phenotype, respectively. The black dot indicates the 5′ end of the P element. See Materials and Methods for detailed descriptions of the screens used to recover these lines and of the techniques used to confirm the presence of a single intact P element in each line and to map its position. In cases where a line has been recovered with a P element inserted within a gene, the gene (in green) is indicated by an asterisk. In all cases tested, the variegating phenotype is suppressed by a mutation in HP1; representative examples are shown in the pictures at the bottom, with the line crossed to the y w67c23 line (the left-hand picture of each pair) compared to the line crossed to the y w67c23, Su(var)2-502 line (the right-hand picture of each pair).

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