FIG. 5.

ChIp analysis reveals GC-induced histone H3 and H4 acetylation of the −2.5 Tat GRU. (A) The amount of immunoprecipitated material from sonicated cross-linked chromatin was quantified using real-time quantitative PCR relatively to a standard curve of genomic DNA. The results shown are means and standard deviations from two immunoprecipitation experiments with duplicate quantification. The −2.5 GRU primers used correspond to nucleosome position N4 in Fig. 4. (B) The amount of immunoprecipitated material from cross-linked chromatin digested with MNase is expressed relative to the input chromatin DNA.