FIG. 6.

TsA-induced histone hyperacetylation triggers neither chromatin remodeling nor HNF3 recruitment. (A) Western blot analysis of acetylation of histone H3 and H4 induced by different TsA treatments, using antibodies against acetylated histone H3 or H4. (B) Chromatin accessibility at the −2.5 Tat GRU as assessed by sensitivity to XbaI or DNase I cleavage. Cells were treated with 100 ng of TsA/ml for 24 h. (C) High-resolution DNase I cleavage analysis of the GC-induced remodeling, revealing the absence of effect of TsA-induced hyperacetylation. The stars on the right indicate the DNase I hyperreactivities characteristic of the GC-induced remodeling, and the arrows indicate those that are characteristic of HNF3 recruitment. The extent of the zone of GC-induced remodeling is shown.