Skip to main content
. 2004 Sep;24(18):7949–7957. doi: 10.1128/MCB.24.18.7949-7957.2004

FIG. 1.

FIG. 1.

PGRP-L is a secreted serum protein. (A) HEK 293 cells were transfected with PGRP-L-myc (lanes 3, 4, 7, and 8) or PGRP-S-myc (lanes 1, 2, 5, and 6). Brefeldin A treatment (lanes 2, 4, 6, and 8) was used for 12 h to block protein secretion. Total cell lysates and culture supernatants were blotted with anti-Myc antibodies. (B) Drosophila Schneider cells were stably transfected with pAC5.1PGRP-L (lane L) or pAC5.1PGRP-S (lane S) or were mock transfected (lane C). Total cell lysates and culture supernatants were blotted with anti-V5 antibodies. (C) Mouse serum or PGRP-L-containing Schneider cell culture supernatants (lane L) were subject to immunoprecipitation with rat anti-PGRP-L antibodies. Immunoprecipitates were blotted with rabbit anti-PGRP-L. (D) HEK 293 cells were cotransfected with PGRP-L-myc and PGRP-L-V5 or PGRP-S-myc and PGRP-L-V5 or were mock transfected. Culture supernatants were incubated with anti-Myc antibodies. Immunoprecipitates were blotted with anti-V5 antibodies.