FIG. 5.

NF-κB activity is increased in MMTV βTrcp1 transgenic mice. (A) DNA binding activity of NF-κB in mammary gland (MG), lachrymal gland (LG), salivary gland (SG), and uterine horn (U) (a). NF-κB activity during the development of mammary gland (virgin mice at 10 weeks and 6 and 22 months of age) and in a breast tumor (MG tumor) in an MMTV βTrcp1 transgenic mouse (b). (B) Nuclear expression of p65/RelA in mammary glands of 10-week-old wild-type and MMTV βTrcp1 transgenic mice. Arrows show representative immunopositive cells with nuclear staining. (C) Extracts from mammary glands of two representative 10-week-old wild-type (WT) and two βTrcp1 transgenic (TG) mice were subjected to immunoblotting with the indicated antibodies (keratin 18 is shown as a control for the epithelial content of the sample).