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. 2004 Sep;24(18):7976–7986. doi: 10.1128/MCB.24.18.7976-7986.2004

FIG. 4.

FIG. 4.

Tgs1p and the conserved KKD/E domain of snoRNP proteins are required for intact nucleolar morphology. (A) EM analysis of strains with TGS1 deleted (tgs1Δ), the catalytic mutants (D103A and W178A), and a wild-type isogenic control (TGS1). For convenience, the wild-type panel was duplicated and the nucleolar structure is outlined with a thin dashed line. No, nucleolus; Np, nucleoplasm; F, fibrillar strands; G, granular component. Bars, 0.25 μm. (B) EM analysis of strains lacking the carboxyl-terminal KKD/E domain of Nop56p (NOP56ΔK) or Nop58p (NOP58ΔK) and a wild-type isogenic control. In NOP56ΔK and NOP58ΔK, a residual nucleolar structure can be detected to the left of each cell. Note that the experiments for panel A were performed at 16°C; identical results were obtained with cells grown at 30°C (data not shown).