FIG. 5.

Nop1p shows a wider distribution in tgs1Δ cells. Indirect immunofluorescence (A) and EM immunogold labeling (B) with a Nop1p-specific antibody are shown. The tgs1Δ strain and a wild-type isogenic control were grown to mid-log phase at 30°C and processed for indirect immunofluorescence or EM immunogold labeling according to standard procedures (see Materials and Methods). (A) The bulk of the DNA was counterstained with DAPI. Cell were observed with a Axioplan2 (Zeiss) microscope and a charge-coupled device-cooled camera. (B) For convenience, the boundary between the nucleolus (No) and the nucleoplasm (Np) in the wild type is outlined with a dotted line and gold particles are circled in red. Bar, 0.25 μm.