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. 2004 Sep;24(18):8227–8235. doi: 10.1128/MCB.24.18.8227-8235.2004

FIG. 4.

FIG. 4.

Swi/Snf silences mURA3 and URA3 in the rDNA. (A) Swi/Snf silences expression of mURA3 in the rDNA. Tenfold serial dilutions of stationary-phase cultures of SNF2 (L1081 and L1084) or snf2Δ (L1079) strains containing the mURA3-LEU2 cassette at the rDNA and SNF2 (L1082 and L1083) or snf2Δ (L1085 and L1086) containing the mURA3-LEU2 cassette at leu2Δ1 were spotted onto 5-FOA and YPD media to monitor expression of mURA3. Loss of silencing is indicated by less growth on 5-FOA. (B) Swi/Snf represses the transcription of URA3 in the rDNA. URA3 mRNA levels were measured in four strains by Northern hybridization analysis. For URA3 at its natural location, FY78 (wild type; lanes 1 and 2) and FY328 (snf2Δ; lanes 3 and 4) were used. For URA3 in the rDNA, FY2313 (wild type; lanes 5 and 6) and FY2316 (snf2Δ; lanes 7 and 8) were used. Strains were grown under conditions both repressing (YPD) and depressing (shifted to 0.05% glucose) for SUC2 transcription (see Materials and Methods). In low glucose, URA3 at its natural location is transcribed at a low level whereas URA3 in the rDNA is not significantly affected. ACT1 served as a loading control.