TABLE 2.
Deletion of SNF2 reduces mitotic recombination in the rDNA
| Relevant genotypea | No. of Ura auxotrophs/total no. of cells analyzed | No. of URA3 markers lost/generationb | Mutant loss rate/ wild-type loss rate |
|---|---|---|---|
| Wild type | 144/4132 (0.035%) | 2.9 × 10−4 | |
| sir2Δ | 2288/5261 (0.43%) | 3.6 × 10−3 | 12.4 |
| snf2Δ | 4/6412 (6.2 × 10−4) | 5.1 × 10−6 | 0.02 |
| snf2Δ sir2Δ | 21/3099 (6.8 × 10−3) | 5.6 × 10−5 | 0.19 |
a
The strains used for these experiments were FY2313, wild type; L1075, sir2Δ; FY2316, snf2Δ; and L1076 and L1077, snf2Δ sir2Δ.
b
The rate of mitotic recombination was determined by measuring the rate of loss of the URA3 marker (number of Ura− auxotrophs/total number of cells analyzed) after 120 generations of growth in nonselective medium (as described in Materials and Methods).