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. 2016 Dec 12;6:39032. doi: 10.1038/srep39032

Figure 1. Small-molecule compounds reactivate HIV latency in a dose-dependent manner in J-Lat-Tat-GFP cells in vitro.

Figure 1

(a) Representative histogram of CD69 and GFP expression in cells in the presence or absence of PMA stimulation for 24 hrs; (b) Levels of cell survival, CD69 and GFP expression in control or PMA stimulated cells. *P < 0.05. The small-molecule compounds include PEP005 (c), prostratin (d) and bryostatin-1 (e), and JQ1 (f). Cells (5 × 105/well) were cultured in the presence of different concentration of each compound for 24 hrs. Cell survival, the early cell activation marker CD69, and GFP expression were analyzed. Note the effects of PEP005 treatment alone on HIV latency reactivation were identical to that of PMA stimulation. (g) The levels of cell survival, CD69 and GFP expression in presence of optimal concentration of PKC activators in combination, with or without JQ1. These data are representative of at least three independent experiments.