Figure 1. 3D culture environment enhances miR combo mediated reprogramming at the genetic level.

(A) Schematic of the experimental protocol. Neonatal cardiac fibroblasts were transfected with negative control miR (negmiR) or miR combo. Two days after transfection cells were re-plated either in regular culture dishes (2D) or encapsulated in a 3D hydrogel (3D). Cells were cultured for a further 14 days and cardiac gene expression analyzed by qPCR. (B) Comparisons of gene expression between 2D and 3D negmiR or miR combo groups (N = 5 independent transfections). *P < 0.05, **P < 0.005.