Figure 3. miR combo mediated induction of αMHC expression is enhanced by 3D culture environment.

Representative FACS analysis (for CFP+ cells) and immunostaining images of neonatal cardiac fibroblasts from αMHC-CFP mice transfected with miR combo and cultured for 14 days in 2D or 3D environment (2D control were untransfected cells). N = 4 independent transfections. ***P < 0.001 between 2D and 3D.