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. 2016 Dec 12;6:38815. doi: 10.1038/srep38815

Figure 6. Effects of 3D environment upon miR combo treatment are MMP-mediated.

Figure 6

Neonatal cardiac fibroblasts were transfected with negative control miR (negmiR) or miR combo. Two days after transfection cells were re-plated either in regular culture dishes (2D) or encapsulated in a 3D hydrogel (3D). Cells were cultured for a further 14 days in the presence of vehicle or 5 uM BB94 (a broad spectrum MMP inhibitor). Cells were immunostained with antibodies for α-Sarcomeric actinin. (A) Representative images, scale bar 100 microns, (B) Quantification, N = 3 independent transfections. For each independent transfection two 3D bundles were stained.*P < 0.05 between BB94 treatment and control in miR combo group.