TABLE 1.
Primers used in this study
| Primer | Sequence (5′→3′) | Generated derivative(s) or use |
|---|---|---|
| SART1-S3014-NcoI | AAAAAACCATGGGCAGCAGCCCTTATCATATACTAC | ORF2+3′UTR |
| SART1-S6222-NotI | AATAATAATTGCGGCCGCGGACCGTCGGGCG | SART1 WT (+NotI)-pAcGHLTB |
| SART1-A6600-BglII | AAAAAAAGATCTGGAAGAAACAGGAAGAAGTCG | 1-379, 73-379 |
| SART1-S6501-NotI | AATAATAATAGCGGCCGCTGAACTCAGCCCAGC | 279-461/(A)20 |
| SART1-A6682-BglII | AAAAAAAGATCTGGTATCGATGGGGAATCCC | ΔpolyA |
| SART1-S6294-NotI | AATAATAATTGCGGCCGCGGGCGCTGTGGCTC | 73-379, 73-379/(A)20, 73-295 |
| SART1-A6516-BglII | AAAAAAAGATCTGGGCTGAGTTCAGCTC | 73-295 |
| SART1-A6600+20A-BglII | AAAAAAAGATCT(T)18TGGAAGAAACAGGAAGAAGTCG | 1-379/(A)20, 73-379/(A)20 |
| SART1-A6704-BglII/BamHI | TTTTTTGGATCCAGATCT(T)19GGTATCGATGGGGAATC | 279-461/(A)20 |
| SART1-S6517 | CGCGCCTTTTTCAAGGCGTAGTCTCC | Δ163-297, Δ228-297 |
| SART1-A6293 | TATACCCTCACCACCACCACTGGACTATCG | Δ73-162, Δ73-227 |
| SART1-S6384 | TGTGGGGGGCCTGCGGGG | Δ73-162, Δ73-227 |
| SART1-S6449 | GAGCTCGTTGGGTTTTAGTCGGTAGTCGTTAAG | Δ73-227, Δ163-227 |
| SART1-A6383 | CCGGCTCCCAGCCTGACGAAC | Δ163-227, Δ163-297 |
| SART1-A6448 | CTATCTTTCCGGCATAGGGGGAACCTACGATAC | Δ228-297 |
| pAcGHLTB-S2183 | CCTATAAATACGGATCTGTATTCATGTCCC | GST-His6 probe |
| pAcGHLTB-A2810 | GGCCATGCTATATACTTGCTGGATTTCAAG | GST-His6 probe |
| pAcGHLTB-S3032 | CGACTCTGCTGAAGAGGAGGAAATTC | Polyhedrin 3′ region probe |
| pAcGHLTB-A3430 | CAAGATTTGGCAAGTTTTGTGGCGTTGAG | Polyhedrin 3′ region probe |
| hsp_pEGFP1-A2330T-S | GCGGCCGCGACTCTAGATCTTAATCAGCCATAC | hsp-EGFP1-BglII |
| hsp_pEGFP1-A2330T-A | GTATGGCTGATTAAGATCTAGAGTCGCGGCCGC | hsp-EGFP1-BglII |
| pEGFP1-S96-EcoRI | AAAAAAGAATTCATGGTGAGCAAGGGCGA | EGFP1/S1-3′UTR-pVL1393 |
| pEGFPI-A901 | GGGGGAGGTGTGGG | EGFP1/S1-3′UTR-pVL1393 |
| pEGFPI-S688 | GACAACCACTACCTGAGCACC | 3′ junction PCR |
| pEGFPI-A576 | GTTCTTCTGCTTGTCGGCCATGATATAG | 5′ junction PCR |