FIG. 4.

Brap2 retains p21 in cytoplasm. HeLa cells were cotransfected with GFP-p21-NLS vector with mock or Brap2 vector. GFP signals were detected by fluorescence microscopy. Photographs in the left columns show cells transfected with mock vector, and those in the right columns show cells transfected with Brap2. Top panels show GFP signals, middle panels show DNA stained with Hoechst 33258, and bottom panels show a phase-contrast view of the corresponding fields. The bar graph shows the mean percentage of cells with GFP signals in the cytoplasm ± standard deviation.