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. 2004 Sep;24(18):8236–8243. doi: 10.1128/MCB.24.18.8236-8243.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 5. — p21 interaction domain on Brap2. pEGFP vectors containing each of the deletion mutant constructs of Brap2, including Brap2 (F; aa 2 to 600), Brap2-N (N; aa 2 to 187), Brap2-M (M; aa 168 to 420), and Brap2-C (C; aa 406 to 600) were transiently cotransfected with pCMV-p21-Myc into HEK293 cells. Cell lysates from each sample were immunoprecipitated (IP) by anti-Myc PAb. The left panel shows the expression of each of the GFP-fused Brap2 deletion mutants demonstrated by anti-GFP PAb (upper panel) and the expression of p21-Myc by anti-Myc PAb (lower panel) in whole lysate. The right panel shows the interaction of Myc-tagged p21 and the deletion mutant of Brap2 in cells. The immune complex of Myc-tagged p21 was analyzed by Western blotting (WB) with anti-GFP PAb.