FIG. 6.

Expression of Brap2 mRNA is increased during monocytic differentiation of U937 cells. Brap2 expression was analyzed by RT-PCR before and after monocytic differentiation induction of U937 cells (top gel). RNA was extracted from U937 or HL60 cells cultured with or without VD₃ for 3 days. p21 expression was induced by VD₃ in U937 and HL60 cells, as demonstrated by RT-PCR (middle gel). Actin mRNA was amplified by RT-PCR and shown as an internal control (bottom gel). The bar graph shows the increase (n-fold) of VD₃-induced Brap2 transcript standardized to samples without VD₃ treatment. The percentage of cells that were CD14 positive is shown below the bars.