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Experimental verification of the reversibility of the inhibition via dialysis assays. Enzyme and inhibitor (final concentration 1 μM) were incubated for 5 min, and the reaction mixture was subjected to dialysis with assay buffer using our published dialysis apparatus.15 The residual enzyme activity was measured after 30, 60, 90, and 120 min by adding the substrate (final concentration 10 μM).
