FIG. 7.

Identification of DH sites encompassing NFAT elements in stably transfected cells. DH sites were assayed in single clones of Jurkat T cells transfected with pHGM0.6 GM-CSF promoter plus chloramphenicol acetyltransferase (CAT) reporter gene plasmids (14) containing either the intact GM-CSF enhancer or three copies of just the NFAT-binding region of the GM420 NFAT site. Cells were either left unstimulated (−) or stimulated for 6 h with 20 ng of PMA per ml and 2 μM A23187 (PMA/I) (+). DH sites were identified by indirect end labeling using a 0.7-kb SacI-ScaI fragment of pHGM0.6 as a probe.