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. 2004 Aug 13;101(34):12485–12490. doi: 10.1073/pnas.0404911101

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Copyright © 2004, The National Academy of Sciences

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Fig. 7. — Oxidation of NADH. NADH was present at 0.1 mM, and oxidation was followed at 340 nm. (A) Line 1, conditions were as in Fig. 1 except as noted below, and was added to 20 mM at the arrow. Line 2, was added to 10 mM at the first arrow, and 0.15 ml of CO₂ solution was added at the second arrow. Line 3, was added to 10 mM at the first arrow, MnSOD was added to 30 μg/ml at the second arrow, and 0.15 ml of CO₂ solution was added at the third arrow. (B) Mn(II) was added at 0.1 mM, H₂O₂ was added at 10 mM, and was added at 10 mM. Line 1, Mn(II) was added at the first arrow, and ethanol to 1% was added at the second arrow. Line 2, reaction was started with H₂O₂ at the first arrow, and then MnSOD was added to 6 μg/ml at the second arrow and to 18 μg/ml at the third arrow. Line 3, as in lines 2 and 3in A but carbonic anhydrase was present at 50 μg/ml. (C) Mn(II) was added at 0.1 mM, H₂O₂ at 10 mM, and , when present, at 5 mM. Line 1, was added at the first arrow, and 0.15 ml of CO₂ solution was added at the second arrow. Line 2, was added at the first arrow, carbonic anhydrase to 50 μg/ml was added at the second arrow, and 0.15 ml of CO₂ solution was added at the third arrow. Line 3, 0.15 ml of CO₂ solution was added at the arrow, but was not present.

Inline graphic — Oxidation of NADH. NADH was present at 0.1 mM, and oxidation was followed at 340 nm. (A) Line 1, conditions were as in Fig. 1 except as noted below, and was added to 20 mM at the arrow. Line 2, was added to 10 mM at the first arrow, and 0.15 ml of CO₂ solution was added at the second arrow. Line 3, was added to 10 mM at the first arrow, MnSOD was added to 30 μg/ml at the second arrow, and 0.15 ml of CO₂ solution was added at the third arrow. (B) Mn(II) was added at 0.1 mM, H₂O₂ was added at 10 mM, and was added at 10 mM. Line 1, Mn(II) was added at the first arrow, and ethanol to 1% was added at the second arrow. Line 2, reaction was started with H₂O₂ at the first arrow, and then MnSOD was added to 6 μg/ml at the second arrow and to 18 μg/ml at the third arrow. Line 3, as in lines 2 and 3in A but carbonic anhydrase was present at 50 μg/ml. (C) Mn(II) was added at 0.1 mM, H₂O₂ at 10 mM, and , when present, at 5 mM. Line 1, was added at the first arrow, and 0.15 ml of CO₂ solution was added at the second arrow. Line 2, was added at the first arrow, carbonic anhydrase to 50 μg/ml was added at the second arrow, and 0.15 ml of CO₂ solution was added at the third arrow. Line 3, 0.15 ml of CO₂ solution was added at the arrow, but was not present.