Table 1. Chemically denatured crosslink-free and prosthetic-group-free proteins.
| Protein | Length | RG,* Å | Conditions | Ref. |
|---|---|---|---|---|
| GroEL | 549 | 82 ± 4 | 4 M urea | 56 |
| yPGK | 416 | 71 ± 1 | 2 M GuHCl | 57 |
| Creatine kinase | 380 | 46.0 ± 1.5 | 6 M GuHCl | This work |
| α-TS | 268 | 48.8 ± 1.0 | 6 M GuHCl | This work |
| Carbonic anhydrase | 260 | 59 ± ≈2 | 6 M GuHCl | 28† |
| OspA | 257 | 49.3 ± 1.9 | 6 M GuHCl | This work |
| DHFR | 167 | 44 ± 2 | 8 M urea | 54 |
| Apomyoglobin | 154 | 40 ± ≈2 | 6 M GuHCl | 28† |
| Snase | 149 | 37.2 ± 1.2 | 6 M GuHCl | This work |
| Lysozyme, reduced | 129 | 35.8 ± 0.5 | 4 M GuHCl | 53 |
| CheY | 129 | 38.0 ± 1.0 | 5-7 M urea | 2 |
| RNase A, reduced | 124 | 33.2 ± 1.0 | 3.25-6 M GuHCl | This work |
| pI3K SH2 | 112 | 29.6 ± 3.3 | 3 M GuHCl | This work |
| pI3K SH3 | 103 | 30.9 ± 0.3 | 2.67 M GuHCl | This work |
| mAcP | 98 | 30.4 ± 1.3 | 6.5-8 M urea | 27 |
| ctACP | 98 | 30.5 ± 0.4 | 5.5-6.8 M urea | This work |
| Protein L | 79 | 26.0 ± 0.6 | 4-5 M GuHCl | 37 |
| Fyn SH3 | 78 | 25.7 ± 0.5 | 6 M GuHCl | This work |
| Ubiquitin | 76 | 25.2 ± 0.2 | 4.9-6 M GuHCl | This work |
| GCN4-p2′ | 66 | 24.1 ± 0.9 | 4.2-6 M GuHCl | This work |
| drK SH3 | 59 | 21.9 ± 0.5 | 2 M GuHCl | 38 |
| Protein G | 52 | 23 ± 1 | 2.3 M GuHCl | 58 |
| N-terminal cytochrome c | 39 | 18.4 ± 1.0 | 4 M urea | This work |
| AK-37 | 37 | 16.9 ± 0.6 | 6 M GuHCl | This work |
| AK-32 | 32 | 14.5 ± 0.6 | 6 M GuHCl | This work |
| AK-27 | 27 | 12.8 ± 0.6 | 6 M GuHCl | This work |
| AK-16 | 16 | 9.8 ± 0.6 | 4 M urea | This work |
| Angiotensin | 8 | 9.1 ± 0.3 | 4 M urea | This work |
*
Measured or estimated sample standard deviations are indicated. These were derived by using a variety of approaches and widely varying numbers of observations and therefore provide, at best, only a qualitative indicator of experimental precision.
†
Solvent conditions and approximate sample standard deviations (G. Semisotnov, personal communication).