Table 5. sel-10(ar41) fails to suppress the feminization of X0 animals caused by If mutations in fem-1, fem-2, and fem-3.
| % CEMs in X0 (n)
|
||
|---|---|---|
| Genotype | +/+ | sel-10(ar41) |
| +/+* | 91 (80) | 91 (100) |
| fem-1(hc17)* | 51 (100) | 53 (112) |
| fem-2(b245)* | 73 (100) | 75 (220) |
| +/+† | 91 (80) | 89 (156) |
| fem-3(e2006)† | 55 (176) | 55 (92) |
The presence of CEMs in X0 animals was scored as described in Materials and Methods. The complete genotypes of the animals analyzed were as listed save that all strains contain him-1(e879) and all strains containing sel-10(ar41) are homozygous for lon-3(e2175).
Animals were cultured at 25°C until reaching the second larval stage, and then the temperature was shifted to 16°C.
Animals were cultured at 20°C.