Figure 6. Apparent heterogeneity in matrix accumulation is cell type and timescale dependent.

(a,b) Representative chondrocytes and MSCs labeled with HPG continuously (a; blue) or between days 7–9 (b; orange). Pulse labeled samples were imaged with higher gain settings than continuously labelled samples, given that the shorter labeling period results in much lower overall intensity (see Fig. S7 for direct comparison). (c,d) Quantified matrix distribution of individual cells, represented as the cumulative fraction of total intensity versus distance from the cell. Median is shown in black; representative cells from (a) and (b) are shown in dashed blue and orange lines. Other individual cells are represented in grey (n = 20 cells/group).