Table 2. Specific activities and kinetic constants of Mpr1 toward AZC and P5C.

Substrate	Specific activity, milliunits/mg	Kcat, sec-1	Km, mM	Kcat/Km, sec-1·mM-1
AZC	24.0 ± 1.49	17.9 ± 0.64	0.90 ± 0.07	19.8 ± 0.69
P5C	14.8 ± 1.97	121 ± 8.80	7.01 ± 0.84	17.2 ± 1.80

Assays were performed in 50 mM Tris·HCl (pH 8.5) for AZC and in 50 mM 2-morpholinoethanesulfonate (pH 7.0) for P5C at 30°C with 0.29 μg of purified enzyme. The values are mean ± standard deviation from three independent experiments.