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. 2013 Nov 14;2:13. doi: 10.1186/2194-0517-2-13

Figure 6.

Figure 6

The osteogenic activity of MG 63 cells cultured on the TCP and scaffolds. (A) ALP activity of MG 63 cells cultured on the TCP and scaffolds after 1, 3, 7, 10 and 14 days. Absorbance was measured at 405 nm. Values are mean ± SD, n = 8. The data were statistically analyzed with a t test. **p < 0.001, there was a significant difference of ALP activity between MG 63 cells seeded on TCP and scaffolds at each time point. *p < 0.05, there was a significant difference of ALP activity between MG63 cells seeded on microscaled scaffolds (16%PCL, 18%PCL) and nanoscaled scaffolds (18%PCL + Pluronic, 16%PCL + PBS) on day 10, and between 16%PCL and the other three scaffold on day 14. (B) MG63 cells seeded in scaffolds and on TCP were fed by standard medium and inductor medium after 1 week culture. The calcium deposition was measured by quantification of o-cresolphthalein complexone after 3 and 4 weeks. Optical density was measured at 570 nm. Values are means ± SD, n = 3.