Table 1. Structural statistics for NMR solution structure of HRV-14 cre stem-loop.
No. of restraints used for structure calculations* | |
NOE-derived distance restraints | 454 |
Interresidue | 175 |
Intraresidue | 279 |
Stem region (1-10, 25-34) | 345 |
Loop region (11-24) | 109 |
Dihedral restraints | 142 |
Hydrogen bond restraints | 52 |
rms deviations from experimental restraints | |
Distance restraints, Å | 0.06 ± 0.003 |
Dihedral restraints, ° | 0.89 ± 0.07 |
Deviations from idealized covalent geometry | |
Bonds, Å | 0.004 ± 0.0001 |
Angles, ° | 1.24 ± 0.01 |
Heavy atom rms deviations (Å) relative to the mean structure | |
All residues | 1.42 ± 0.31 |
Loop region (residues 11-24) | 1.97 ± 0.37 |
Stem region (residues 1-10; 25-34) | 1.15 ± 0.16 |
The final structures contained fewer than three distance restraint violations >0.2 Å and no dihedral angle violations per structure.